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Table 2 qPCR primers used in this study

From: Testicular expression of TDRD1, TDRD5, TDRD9 and TDRD12 in azoospermia

Gene

Primers (5′ → 3′)

Product size (bp)

TM (°C)

TDRD1

F: TCCTCTTCGGTCCACAACTT

197

59

R: CCTCCACATCCTTTGTTTCAA

 

60

TDRD5

F: AAGTTCCCAGAGGGTTTGTTT

194

58

R: AGAGGCTTCTTATCCGCAT

 

59

TDRD9

F: GCCAGGTCTGGGTGAAATAA

171

58

R:TCTGCAATATTGGTGGACAGA

 

58

TDRD12

F: TCGTTTATGCAGCTTCCCTA

172

57

R: CCACCTGGGTAGTTGCTTT

 

58

ACTB

F: AGCACAGAGCCTCGCCTT

172

60

R: AGGGCATACCCCTCGTAGAT

 

60

  1. bP Base pairs, F Forward, R Reverse, TM Melting temperature