Table 3 Primer sequences, PCR size and PCR-RFLP fragments using AIWNI and NIaIII restriction enzymes for MUC1 5640G > A (rs4072037) and PSCA 5057C > T (rs2294008) polymorphisms, respectively
SNP | Primer sequence | PCR Size (bp) | Restriction enzyme | Digestion products (bp) | ||
|---|---|---|---|---|---|---|
| Â | Â | Â | Â | Wild-type | Mutant | Heterozygous |
MUC1 5640G > A (rs4072037) | F: TAAAGACCCAACCCTATGACT | 188 | AIWNI | 188 | 114,74 | 188,114,74 |
R: AGAGTACGCTGCTGGTCATACTC | ||||||
PSCA 5057C > T (rs2294008 | F: GAAACCCGCTGGTGTTGACT | 139 | NIaIII | 139 | 101,38 | 139,101,38 |
R: GCCAAGCCTGCCATCAACAG | ||||||