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Table 1 PCR protocol

From: Identification of ATP2C1 mutations in the patients of Hailey-Hailey disease

94 °C 5 min

I

94 °C 45 s

60 °C 45 s

72 °C 45 s

2×

1

94 °C 45 s

59 °C 45 s

72 °C 45 s

2×

94 °C 45 s

58 °C 45 s

72 °C 45 s

2×

94 °C 45 s

57 °C 45 s

72 °C 45 s

2×

94 °C 45 s

56 °C 45 s

72 °C 45 s

2×

94 °C 45 s

55 °C 45 s

72 °C 45 s

20×

II

94 °C 45 s

55 °C 45 s

72 °C 45 s

32×

Other exon

III

94 °C 30s

54 °C 30s

72 °C 30s

30×

5, 7, 12, 27, 28

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BMC Medical Genetics

ISSN: 1471-2350

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