Table 1 Primers for PCR and Sanger sequencing used in mutation validation
Gene | Transcript | mutation | Forward primer (5′-3′) | Reverse primer (5′-3′) | Product length (bp) |
|---|---|---|---|---|---|
GJA8 | NM_005267.4 | c.154 T > C | GCAACTTGGAAAGGAGAGGT | ATGTGGCAGATGTAGGTCCT | 576 |
GJA3 | NM_021954.3 | c.1152_1153insG | GATGACTGAGCAGAACTGGG | CCTGATCTCTCCTCCATCGT | 496 |
BFSP1 | NM_001195.3 | c.1804G > C | CCAATTGACCAGCAGCCTAT | CTGTCCTCATGAAGCTGACC | 623 |
EPHA2 | NM_004431.3 | c.1532C > T | AATTCCGAGCCTCAGTTTCC | TGAACTTCCTCACACCACTG | 540 |
HSF4 | NM_001538.3 | c. 356G > A | CTGCCCCAGTATTTCAAGCA | CCTCCTCTTTGCTCATTCCC | 309 |