Table 2 Primers for PCR amplification and Sanger-sequencing of exons and flanking regions of NPC1 and NPC2 genes
Exon # | Primer | PCR fragment size | Primers Tm |
|---|---|---|---|
1 | NPC1F 5′-GAGCCAGACTCCATAAGTC-3′ NPC1R 5′-AGACCAACTTCCCCAGGAC-3′ | 466 | 64 |
2 | NPC2F 5′-GATTGTACTTGAGTGGGCAC-3′ NPC2R 5′-ACAGAGGATCTTGTGATCAG-3′ | 238 | 62 |
3 | NPC3F 5′-TGAGGAATGTTGACCTTACTCTAAC-3′ NPC3R 5′-GAAAGCTGAGCATTACCAGTTC-3’ | 207 | 64 |
4 | NPC4F 5’-GCTGGCCCTATTATGTGTGAG-3′ NPC4R 5′-ATTTCCTGGCCAATGGAACTG-3’ | 312 | 64 |
5–6 | NPC5F 5’-CAGCATTCCAGCATGGTGCATATG-3′ NPC6R 5′-CCATGCAATGGTATTCATGGAGG-3’ | 1126 | 64 |
7–8 | NPC7F 5’-GAAGGCAGTAATTAGGGAGG-3′ NPC8R 5′-CCACAAGGTCATCTAGAGTG-3’ | 1108 | 62 |
9 | NPC9F 5’-GCTGATTAATCAAGATCTGAGAG-3′ NPC9R 5′-CTCACCTCTGGGTTATGCTC-3’ | 367 | 64 |
10 | NPC10F 5’- GCTGAGCTGTATTACTCAACTG-3′ NPC10R 5′-TACCACTTGATGCTAATGAC-3’ | 292 | 64 |
11 | NPC11F 5’- CAGAGATACAGTCCATAGCTC-3′ NPC11R 5′- GAGCTGAGATTCAGTCACTG-3’ | 501 | 62 |
12–13 | NPC12F 5’- CTTTGTATCGTGAAAGTTAG-3′ NPC13R 5′-CCAGGAGCCATTCACAGTC-3’ | 931 | 60 |
14 | NPC14F 5’- CTGCTGTAGAAGGTGGTCTC-3′ NPC14R 5′- GACATGTTCAGGTAGCCAGC-3’ | 507 | 64 |
15–16 | NPC15F 5’-CTTGTATCTGTACATGCACATG-3′ NPC16R 5′-GATAATCTGTTTCAGTGAGAGG-3’ | 472 | 64 |
17 | NPC17F 5’-GCCCTGTACTCCCTATTAGC-3′ NPC17R 5′-GTTAGAAGCAGGCACTTGCTT-3’ | 298 | 62 |
18–19 | NPC18F 5’-GAATCATGAGTCCAGCTGGAG-3′ NPC19R 5′-GGGAGACCCAGCTTTGATATAC-3’ | 876 | 62 |
20 | NPC20F 5’- GAAAGTGACATGTGGCTGAAG-3′ NPC20R 5′- GTGGATGCTTATCTGCAATGGC-3’ | 315 | 60 |
21 | NPC21F 5’- CAAGACCTGGACTCTCTTGAC-3′ NPC21R 5′- GATATACTGCCCTGTGCTCAG-3’ | 357 | 62 |
22 | NPC22F 5’- AGGAGTCTGACCACTTGGCAGT-3′ NPC22R 5′- ACATGGAATCTAAGACAGCC-3’ | 382 | 64 |
23 | NPC23F 5’-GAGGCCTTGTAAGTCCAATGGG-3′ NPC23R 5′-GTACAGGATCCAGACTCTTCAG-3’ | 312 | 64 |
24 | NPC24F 5’- GAGAAATCCTTGTAAGGAAG-3′ NPC24R 5′- GATGAGAACTCTTACCTATG-3’ | 228 | 64 |
25 | NPC25F 5’-TTCCAAAGTGGGATTACAGGCGTG-3′ NPC25R 5′-GACCGACCCTTAGACACAGTTCAG-3’ | 183 | 64 |
1 | NC2-1F 5’-AGACTGCAGGCTTCTGGGCCTGAG-3′ NC2-1R 5′-CCAGCCCCAGGGGTCTCAGCGC-3’ | 332 | 64 |
2 | NC2-2F 5’-AGCAGAGCACCTTCCCATTAG G-3′ NC2-2R 5′-CTCCCCTCCATTCCCATGCTT A-3’ | 256 | 64 |
3 | NC2-3F 5’-ATGCTGTTGCTTGGGATTATTTC-3′ NC2-3R 5′-CCCATCTCTGCTTCTTGCCCACT-3’ | 339 | 62 |
4 | NC2-4F 5’-GGCTGTAAGCTGTGCCCACATGCT-3′ NC2-4R 5′-CTGGACCTTCCTTACTCCGACAG-3’ | 552 | 62 |
5 | NC2-5F 5’-TAACTTGCCCTAGGGTTATTGC- 3′ NC2-5R 5′-GTGCACTCTGGGACCACGGAACT-3’ | 505 | 62 |