Figure 5
From: Lamin A/C truncation in dilated cardiomyopathy with conduction disease
Digest with AlwN I to confirm the 908_909delCT mutation. The 908_909delCT mutation creates a novel AlwN I restriction site. Exon 5 was amplified from the proband and control genomic DNA and subsequently digested with AlwN I. Where control DNA lacks an AlwN I site (294 bp), the proband's exon 5 was digested with AlwN I generating fragments of 194 bp and 119 bp. DNA from 100 ethnically-matched control chromosomes was similarly checked for this mutation and did not have the 908_909delCT mutation. Lane 1, control; Lane 2, proband; Lane 3, size markers.