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Table 2 Primer used to amplify the dopamine receptor, beta-actin and their competitive mutants

From: Change of dopamine receptor mRNA expression in lymphocyte of schizophrenic patients

   

product

Target

Primer

primer sequence

length (bp)

D3R

Upstream

5'-ACG-ACA-TGG-CTG-GGC-TAC-G-3'

155

 

Downstream

5' -GCC-AAC-AGC-CTG-GCG-CTA-G-3'

 

D5R

Upstream

5'-GTC-GCC-GAG-GTG-GCC-GGT-TAC-3'

362

 

Downstream

5'-GCT-GGA-GTC-AGA-ATT-CTC-TGC-AT-3'

 

BA

Upstream

5'-CGT-GGG-CCG-CCC-TAG-GCA-CCA-3'

260

 

Downstream

5'-TTG-GCC-TTA-GGG-TTC-AGG-GGG-G-3'

 

Mutant D3R

Upstream

5' -TCA-AGA-GTT-CCC-TAT-CAC-TCT-CAA-GTT-

474

  

TCG-TGA-GCT-GAT -TG-3'

 
 

Down stream

5'-GTC-GCC-GAG-GTG-GCC-GGT-TAC-CAA-GTT-

 
  

TCG-TGA-GCT-GAT -TG-3'

 

Mutant D5R

Upstream

5'-GTC-GCC-GAG-GTG-GCC-GGT-TAC-CAA-GTT-

435

  

TCG-TGA-GCT-GAT -TG-3'

 
 

Downstream

5'-GCT-GGA-GTC-AGA-ATT-CTC-TGC-ATA-TTT-

 
  

GAT-TCT-GGA-CCA-TGG-C-3'

 

Mutant βA

Upstream

5'-TTG-GCC-TTA-GGG-TTC-AGG-GGG-GAT-TTG-

429

  

ATT-CTG-GAC-CAT-GGC-3'

 
 

Downstream

5'-CGT-GGG-CCG-CCC-TAG-GCA-CCA-CAA-GTT-

 
  

TCG-TGA-GCT -GAT-TG-3'